Mayor Sadiq Khan has told people they must stop using the London Underground for non-essential use.
He shared a video on Twitter that read: "Let me be clear: Londoners must stop all non-essential use of public transport now.
"This is a matter of public safety."
Brits had been told to stop all non-essential travel over the coronavirus outbreak this week.
However, images surfaced on social media continuing to show crowds gathering at tube stations.
The Foreign Office has told Brits to avoid travelling out of the country because they will be at risk of failing to get back.
Scores of Londoners are now working from home and no longer need the service.
In the UK, there have been 144 deaths so far and more than 3,000 cases after the disease broke out in Wuhan, China, at the end of last year.
Earlier today, it emerged paracetamol was being sold on eBay for £2,550.
Matthew Allen, a disabled dad, told the Sun Online: "The people robbing the needy to make a little extra cash when it could mean people lives is criminal.
Colouring in pictures to keep your kids entertained during coronavirus isolation
"The people stockpiling because they are panicked, ok I get it, but at least it's to keep them and family safe especially if people are to be isolated you then know you have stuff in.
"But I was disgusted to see people making money off the back of vulnerable and scared people whether its masks, sanitiser or tablets.
"It shows that we are not Great Britain any more and the people that lived and died within world war 2 would be turning in their graves to see how selfish the generations are that where given a life by these people that sacrificed so much."
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